Changes in antifungal defence systems during the intermoult period in the Colorado potato beetle

Tomilova, O. G., O. N. Yaroslavtseva, M. D. Ganina, M. V. Tyurin, E. I. Chernyak, I. V. Senderskiy, Y. A. Noskov, O. V. Polenogova, Y. B. Akhanaev, V. Yu. Kryukov, V. V. Glupov, S. V. Morozov. Journal of Insect Physiology 116: 106-117. https://doi.org/10.1016/j.jinsphys.2019.05.003

Susceptibility to the fungus Metarhizium robertsii and changes in host defences were evaluated in different stages of the intermoult period (4–6 h, 34–36 h and 84–86 h post moult in IV larval instars) of the Colorado potato beetle. A significant thickening of the cuticle during larval growth was accompanied by decreases in cuticle melanization, phenoloxidase activity and epicuticular hydrocarbon contents (C28-C32). At the same time, a decrease in the conidial adhesion rate and an increase in resistance to the fungus were observed. In addition, we recorded significant elevation of the encapsulation rate and total haemocyte counts in the haemolymph during the specified period. The activity of detoxification enzymes decreased in the haemolymph but increased in the fat body during larval growth. No significant differences in the fatty acid content in the epicuticle were observed. The role of developmental disorders in susceptibility to entomopathogenic fungi is also discussed.